article Open Access (bronze) EN 2019-10-15

Blind prediction of homo‐ and hetero‐protein complexes: The CASP13‐CAPRI experiment

Marc F. Lensink, Guillaume Brysbaert, Nurul Nadzirin, Sameer Velankar, Raphaël A. G. Chaleil, Tereza Gerguri, Paul A. Bates, Élodie Laine, Alessandra Carbone, Sergei Grudinin, Ren Kong, Ran‐Ran Liu, Ximing Xu, Hang Shi, Shan Chang, Miriam Eisenstein, Agnieszka Karczyńska, Cezary Czaplewski, Emilia A. Lubecka, Agnieszka G. Lipska, Paweł Krupa, Magdalena A. Mozolewska, Łukasz Golon, Sergey A. Samsonov, Adam Liwo, Silvia Crivelli, Guillaume Pagès, Mikhail Karasikov, Maria Kadukova, Yumeng Yan, Sheng‐You Huang, Mireia Rosell, Luis Angel Rodríguez‐Lumbreras, Miguel Romero‐Durana, Lucía Díaz, Juan Fernández‐Recio, Charles Christoffer, Genki Terashi, Woong‐Hee Shin, Tunde Aderinwale, Sai Raghavendra Maddhuri Venkata Subraman, Daisuke Kihara, Dima Kozakov, Sándor Vajda, Kathryn Porter, Dzmitry Padhorny, Israel Desta, Dmitri Beglov, Mikhail Ignatov, Sergey Kotelnikov, Iain H. Moal, David W. Ritchie, Isaure Chauvot de Beauchêne, Bernard Maigret, Marie‐Dominique Devignes, Maria Elisa Ruiz Echartea, Didier Barradas‐Bautista, Zhen Cao, Luigi Cavallo, Romina Oliva, Yue Cao, Yang Shen, Minkyung Baek, Taeyong Park, Hyeonuk Woo, Chaok Seok, Merav Braitbard, Lirane Bitton, Dina Scheidman‐Duhovny, Justas Dapkūnas, Kliment Olechnovič, Česlovas Venclovas, Petras J. Kundrotas, Saveliy Belkin, Devlina Chakravarty, Varsha D. Badal, Ilya A. Vakser, Thom Vreven, Sweta Vangaveti, Tyler Borrman, Zhiping Weng, Johnathan D. Guest, Ragul Gowthaman, Brian G. Pierce, Xianjin Xu, Rui Duan, Liming Qiu, Jie Hou, Benjamin Ryan Merideth, Zhiwei Ma, Jianlin Cheng, Xiaoqin Zou, Panagiotis I. Koukos, Jorge Roel‐Touris, Francesco Ambrosetti, Cunliang Geng, Jörg Schaarschmidt, Mikaël Trellet, Adrien S. J. Melquiond, Li C. Xue, Brian Jiménez‐García, Charlotte W. van Noort, Rodrigo V. Honorato, Alexandre M. J. J. Bonvin, Shoshana J. Wodak

Proteins Structure Function and Bioinformatics, Vol. 87, Issue 12, pp. 1200–1221 (2019)

Centre National de la Recherche Scientifique, Université de Lille, Unité de Glycobiologie Structurale et Fonctionnelle, European Bioinformatics Institute, Wellcome Trust, The Francis Crick Institute, Sorbonne Université, Biologie Computationnelle, Quantitative et Synthétique, Institut de Biologie Paris-Seine, Institut Universitaire de France, Institut polytechnique de Grenoble, Centre Inria de l'Université Grenoble Alpes, Laboratoire Jean Kuntzmann, Université Grenoble Alpes, Jiangsu University of Technology, University of Missouri, Weizmann Institute of Science, University of Gdańsk, Institute of Physics, Institute of Psychology, Polish Academy of Sciences, Institute of Computer Science, Korea Institute for Advanced Study, University of California, Davis, ETH Zurich, Moscow Institute of Physics and Technology, Huazhong University of Science and Technology, Barcelona Supercomputing Center, Instituto de Ciencias de la Vid y del Vino, Universitat Politècnica de Catalunya, Institut de Biologia Molecular de Barcelona, Purdue University West Lafayette, Stony Brook University, Boston University, Laboratoire Lorrain de Recherche en Informatique et ses Applications, Université de Lorraine, King Abdullah University of Science and Technology, Parthenope University of Naples, Texas A&M University, Seoul National University, Hebrew University of Jerusalem, Vilnius University, University of Kansas, University of Massachusetts Chan Medical School, Advanced Bioscience Laboratories (United States), Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, University of Applied Sciences Utrecht, Utrecht University, VIB-VUB Center for Structural Biology
DOI: 10.1002/prot.25838 PMID: 31612567

Abstract

We present the results for CAPRI Round 46, the third joint CASP-CAPRI protein assembly prediction challenge. The Round comprised a total of 20 targets including 14 homo-oligomers and 6 heterocomplexes. Eight of the homo-oligomer targets and one heterodimer comprised proteins that could be readily modeled using templates from the Protein Data Bank, often available for the full assembly. The remaining 11 targets comprised 5 homodimers, 3 heterodimers, and two higher-order assemblies. These were more difficult to model, as their prediction mainly involved "ab-initio" docking of subunit models derived from distantly related templates. A total of ~30 CAPRI groups, including 9 automatic servers, submitted on average ~2000 models per target. About 17 groups participated in the CAPRI scoring rounds, offered for most targets, submitting ~170 models per target. The prediction performance, measured by the fraction of models of acceptable quality or higher submitted across all predictors groups, was very good to excellent for the nine easy targets. Poorer performance was achieved by predictors for the 11 difficult targets, with medium and high quality models submitted for only 3 of these targets. A similar performance "gap" was displayed by scorer groups, highlighting yet again the unmet challenge of modeling the conformational changes of the protein components that occur upon binding or that must be accounted for in template-based modeling. Our analysis also indicates that residues in binding interfaces were less well predicted in this set of targets than in previous Rounds, providing useful insights for directions of future improvements.

Topics

Protein Structure and Dynamics 1.00 Computational Drug Discovery Methods 0.99 Enzyme Structure and Function 0.99

Field: Biochemistry, Genetics and Molecular Biology · Subfield: Molecular Biology

Keywords

CASP,Template,Docking (animal),Protein structure prediction,Computer science,Computational biology,Chemistry,Biology,Protein structure,Biochemistry

MeSH Terms

AlgorithmsAlgorithmsAlgorithmsAlgorithmsAlgorithmsAlgorithmsBinding SitesBinding Sites

UN Sustainable Development Goals

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