article Open Access (gold) EN 2004-09-09

A human in vitro model system for investigating genome-wide host responses to SARS coronavirus infection

Lisa F. P. Ng, Martin L. Hibberd, Eng Eong Ooi, Kin-Fai Tang, Soek-Ying Neo, J. S. Tan, K. R. K. Murthy, Vinsensius B. Vega, Jer-Ming Chia, Edison T. Liu, Ee-Chee Ren

BMC Infectious Diseases, Vol. 4, Issue 1, pp. 34–34 (2004)

Genome Institute of Singapore, National University of Singapore, National Environment Agency, Singapore Science Park

DOI: 10.1186/1471-2334-4-34 PMID: 15357874

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Abstract

BACKGROUND: The molecular basis of severe acute respiratory syndrome (SARS) coronavirus (CoV) induced pathology is still largely unclear. Many SARS patients suffer respiratory distress brought on by interstitial infiltration and frequently show peripheral blood lymphopenia and occasional leucopenia. One possible cause of this could be interstitial inflammation, following a localized host response. In this study, we therefore examine the immune response of SARS-CoV in human peripheral blood mononuclear cells (PBMCs) over the first 24 hours. METHODS: PBMCs from normal healthy donors were inoculated in vitro with SARS-CoV and the viral replication kinetics was studied by real-time quantitative assays. SARS-CoV specific gene expression changes were examined by high-density oligonucleotide array analysis. RESULTS: We observed that SARS-CoV was capable of infecting and replicating in PBMCs and the kinetics of viral replication was variable among the donors. SARS-CoV antibody binding assays indicated that SARS specific antibodies inhibited SARS-CoV viral replication. Array data showed monocyte-macrophage cell activation, coagulation pathway upregulation and cytokine production together with lung trafficking chemokines such as IL8 and IL17, possibly activated through the TLR9 signaling pathway; that mimicked clinical features of the disease. CONCLUSIONS: The identification of human blood mononuclear cells as a direct target of SARS-CoV in the model system described here provides a new insight into disease pathology and a tool for investigating the host response and mechanisms of pathogenesis.

Topics

COVID-19 Clinical Research Studies 0.58 SARS-CoV-2 and COVID-19 Research 0.34 Long-Term Effects of COVID-19 0.02

Field: Medicine · Subfield: Infectious Diseases

Keywords

Immunology,Peripheral blood mononuclear cell,Chemokine,Immune system,Biology,Pathogenesis,Viral replication,Coronavirus,Virology,Medical microbiology

MeSH Terms

AnimalsAnimalsAnimalsChlorocebus aethiopsChlorocebus aethiopsChlorocebus aethiopsHumansHumans

UN Sustainable Development Goals

Good health and well-being (0.76)

All Available Versions

PDF Landing page — BMC Infectious Diseases publishedVersion cc-by
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Landing page — LSHTM Research Online (London School of Hygiene and Tropical Medicine) acceptedVersion
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Landing page — National University of Singapore submittedVersion

Citations by Year

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APA

Lisa F. P. Ng, Martin L. Hibberd, Eng Eong Ooi, Kin-Fai Tang, Soek-Ying Neo, J. S. Tan, K. R. K. Murthy, Vinsensius B. Vega, Jer-Ming Chia, Edison T. Liu, Ee-Chee Ren (2004). A human in vitro model system for investigating genome-wide host responses to SARS coronavirus infection. BMC Infectious Diseases, 4(1), 34–34. https://doi.org/10.1186/1471-2334-4-34

MLA

Lisa F. P. Ng, et al. "A human in vitro model system for investigating genome-wide host responses to SARS coronavirus infection." BMC Infectious Diseases, vol. 4, no. 1, 2004, pp. 34–34. https://doi.org/10.1186/1471-2334-4-34

Chicago

Lisa F. P. Ng, Martin L. Hibberd, Eng Eong Ooi, Kin-Fai Tang, Soek-Ying Neo, J. S. Tan, K. R. K. Murthy, Vinsensius B. Vega, Jer-Ming Chia, Edison T. Liu, Ee-Chee Ren. "A human in vitro model system for investigating genome-wide host responses to SARS coronavirus infection." BMC Infectious Diseases 4, no. 1 (2004): 34–34. https://doi.org/10.1186/1471-2334-4-34

BibTeX

@article{ng2004,
  title = { A human in vitro model system for investigating genome-wide host responses to SARS coronavirus infection },
  author = { Lisa F. P. Ng and Martin L. Hibberd and Eng Eong Ooi and Kin-Fai Tang and Soek-Ying Neo and J. S. Tan and K. R. K. Murthy and Vinsensius B. Vega and Jer-Ming Chia and Edison T. Liu and Ee-Chee Ren },
  journal = { BMC Infectious Diseases },
  year = { 2004 },
  volume = { 4 },
  number = { 1 },
  pages = { 34--34 },
  doi = { 10.1186/1471-2334-4-34 }
}

RIS

TY  - JOUR
TI  - A human in vitro model system for investigating genome-wide host responses to SARS coronavirus infection
AU  - Lisa F. P. Ng
AU  - Martin L. Hibberd
AU  - Eng Eong Ooi
AU  - Kin-Fai Tang
AU  - Soek-Ying Neo
AU  - J. S. Tan
AU  - K. R. K. Murthy
AU  - Vinsensius B. Vega
AU  - Jer-Ming Chia
AU  - Edison T. Liu
AU  - Ee-Chee Ren
JO  - BMC Infectious Diseases
PY  - 2004
VL  - 4
IS  - 1
SP  - 34
EP  - 34
DO  - 10.1186/1471-2334-4-34
ER  -

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References

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